清中研究室 KIYONAKA laboratory, Nagoya University 名古屋大学 大学院工学研究科 生命分子工学専攻

メンバー

堂浦 智裕

学歴

職歴

現在に至る

原著論文

  1. Ojima K., Kakegawa W., Yamasaki T., Miura Y., Itoh M., Michibata Y., Kubota R., Doura T., Miura E., Nonaka H., Mizuno S., Takahashi S., Yuzaki M., Hamachi I., Kiyonaka S. Coordination chemogenetics for activation of GPCR-type glutamate receptors in brain tissue. Nat. Commun.,13, 3167 (2022). DOI: 10.1038/s41467-022-30828-0
  2. Miura Y., Senoo A., Doura T., Kiyonaka S.　Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches.RSC Chem. Biol.,3, 269-287 (2022). DOI: 10.1039/d1cb00195g.
  3. Senoo A., Yamada Y., Ojima K., Doura T., Hamachi I., Kiyonaka S. Orthogonal activation of metabotropic glutamate receptor using coordination chemogenetics. Front. Chem. 9, 825669 (2022). DOI: 10.3389/fchem.2021.825669
  4. Tsai Y.H., Doura T., Kiyonaka S. Tethering-based chemogenetic approaches for the modulation of protein function in live cells. Chem. Soc. Rev. 50,7909-7923 (2021). DOI: 10.1039/d1cs00059d
  5. Ojima K., Shiraiwa K., Soga K., Doura T., Takato M., Komatsu K., Yuzaki M., Hamachi I., Kiyonaka S. (2021) Ligand-directed two-step labeling to quantify neuronal glutamate receptor trafficking. Nature Commun. 12, 831
  6. Aoyama H., Doura T. (2020) Selective acetylcholinesterase inhibitors derived from muscle relaxant dantrolene. Bioorg. Med. Chem. Lett. 30, 126888
  7. Doura T., Nishio T., Tamanoi F., and Nakamura M. (2019) Relationship between the glutathione-responsive degradability of thiol-organosilica nanoparticles and the chemical structures. J. Mater. Res. 34, 1266-1278
  8. Mekaru H., Yoshigoe A., Nakamura M., Doura T., and Tamanoi F. (2019) Biodegradability of disulfide-organosilica nanoparticles evaluated by soft X-ray photoelectron spectroscopy: cancer therapy implications. ACS Appl. Nano Mater. 2, 479-488
  9. Doura T., Tamanoi F., and Nakamura M. (2018) Miniaturization of thiol-organosilica nanoparticles induced by an anionic surfactant. J. Colloid Interface Sci. 526, 51-62
  10. Moriizumi Y., Tabata K. V., Watanabe R., Doura T., Kamiya M., Urano Y., and Noji H. (2018) Hybrid cell reactor system from Escherichia coli protoplast cells and arrayed lipid bilayer chamber device. Sci. Rep. 8, 11757
  11. Doura T., Takahashi K., Ogura Y., and Suzuki N. (2017) Combretastatin A4-b-galactosyl conjugates for ovarian cancer prodrug monotherapy. ACS Med. Chem. Lett. 8, 211-214
  12. Doura T., Kamiya M., Obata F., Yamaguchi Y., Hiyama T. Y., Matsuda M., Fukamizu A., Noda M., Miura M., and Urano Y. (2016) Detection of lacZ-positive cells in living tissue with single-cell resolution. Angew. Chem. Int. Ed. 55, 9620-9624
  13. Doura T., Yamada M., Teranishi R., Yamamoto Y., Sugimoto T., Yuba E., Harada A., and Kono K. (2015) PAMAM dendron lipid assemblies that undergo structural transition in response to weakly acidic pH and their cytoplasmic delivery capability. Langmuir 31, 5105-5114
  14. Nonaka H., An Q., Sugihara F., Doura T., Tsuchiya A., Yoshioka Y., and Sando S. (2015) Phenylboronic acid-based 19F MRI probe for the detection and imaging of hydrogen peroxide utilizing its large chemical-shift change. Anal. Sci. 31, 331-335
  15. Doura T., Hata R., Nonaka H., Sugihara F., Yoshioka Y., and Sando S. (2013) An adhesive 19F MRI chemical probe allows signal off-to-on-type molecular sensing in a biological environment. Chem. Commun. 49, 11421-11423
  16. Nonaka H., Hata R., Doura T., Nishihara T., Kumagai K., Akakabe M., Tsuda M., Ichikawa K., and Sando S. (2013) A platform for designing hyperpolarized magnetic resonance chemical probes. Nat. Commun. 4, 2411
  17. Doura T., Hata R., Nonaka H., Ichikawa K., and Sando S. (2012) Design of a 13C magnetic resonance probe using a deuterated methoxy group as a long-lived hyperpolarization unit. Angew. Chem. Int. Ed. 51, 10114-10117
  18. Doura T., Nonaka H., and Sando S. (2012) Atom arrangement strategy for designing a turn-on 1H magnetic resonance probe: a dual activatable probe for multimodal detection of hypochlorite. Chem. Commun. 48, 1565-1567
  19. Doura T., An Q., Sugihara T., Matsuda T., and Sando S. (2011) p-Aminophenyl alkyl ether-based 19F MRI probe for specific detection and imaging of hypochlorite ion. Chem. Lett. 40, 1357-1359

総説・解説等

  1. 堂浦智裕、神谷真子、浦野泰照 (2016) クローズアップ実験法：生体組織中のlacZ発現細胞のライブ蛍光検出 実験医学12月号 Vol. 34, No. 19, 3197-3201

研究分野

ケミカルバイオロジー、ケミカルジェネティクス、メディシナルケミストリー

  1. Gタンパク質共役型受容体（GPCR）を対象とした化学遺伝学研究
    GPCRは細胞外からの刺激を細胞内へ伝えるGタンパク質シグナルやアレスチンシグナルの最上流に位置し、様々な細胞応答の制御に関わっている。また、同じGPCRでも発現する細胞が異なると生理学的機能が異なる。そのため、細胞別のGPCRの機能解明のための化学遺伝学的な方法論の開発に取り組んでいる。