清中研究室 KIYONAKA laboratory, Nagoya University 名古屋大学 大学院工学研究科 生命分子工学専攻

メンバー

清中 茂樹

略歴

現在に至る

研究分野

研究内容を参考ください

原著論文および総説

原著論文 (Original Paper)

    　
  1. Ojima K., Kakegawa W., Yamasaki T., Miura Y., Itoh M., Michibata Y., Kubota R., Doura T., Miura E., Nonaka H., Mizuno S., Takahashi S., Yuzaki M., Hamachi I., Kiyonaka S. Coordination chemogenetics for activation of GPCR-type glutamate receptors in brain tissue. Nat. Commun., 13, 3167 (2022). DOI: 10.1038/s41467-022-30828-0
  2. Senoo A., Yamada Y., Ojima K., Doura T., Hamachi I., Kiyonaka S. Orthogonal activation of metabotropic glutamate receptor using coordination chemogenetics. Front. Chem., 9, 825669 (2022). DOI: 10.3389/fchem.2021.825669.
  3. Ojima K., Shiraiwa K., Soga K., Doura T., Takato M., Komatsu K., Yuzaki M., Hamachi I., Kiyonaka S. Ligand-directed two-step labeling to quantify neuronal glutamate receptor trafficking. Nature Commun. 12, 831 (2021).
  4. Sakamoto S., Yamaura K., Numata T., Harada F., Amaike K., Inoue R., Kiyonaka S., Hamachi I. Construction of a Fluorescent Screening System of Allosteric Modulators for the GABAA Receptor Using a Turn-On Probe. ACS Cent. Sci. 5, 1541-1553 (2019).
  5. Kubota R., Kiyonaka S., Hamachi I. On-cell coordination chemistry: Chemogenetic activation of membrane-bound glutamate receptors in living cells. Methods Enzymol, 622, 411-430 (2019).
  6. Ha H.T.T., Leal-Ortiz S., Lalwani K., Kiyonaka S., Hamachi I., Mysore S.P., Montgomery J.M., Garner C.C., Huguenard J.R., Kim S.A. Shank and Zinc Mediate an AMPA Receptor Subunit Switch in Developing Neurons. Front. Mol. Neurosci., 11, 405 (2018).
  7. Kubota R., Nomura W., Iwasaka T., Ojima K., Kiyonaka S., Hamachi I. Chemogenetic Approach Using Ni(II) Complex-Agonist Conjugates Allows Selective Activation of Class A G-Protein-Coupled Receptors. ACS Cent. Sci., 4, 1211-1221 (2018).
  8. Kiyonaka S., Sakamoto S., Wakayama S., Morikawa Y., Tsujikawa M., Hamachi I. Ligand-directed chemistry of AMPA receptors confers live-cell fluorescent biosensors. ACS Chem. Biol., 13, 1880-1889 (2018).
  9. Kurokawa T., Kiyonaka S., Nakata E., Endo M., Koyama S., Mori E., Tran N.H., Dinh H., Suzuki Y., Hidaka K., Kawata M., Sato C., Sugiyama H., Morii T., Mori Y. DNA origami scaffolds as templates for functional tetrameric Kir3 K+ channels. Angew. Chem. Int. Ed., 57, 2586-2591 (2018).
  10. Tamura T., Song Z., Amaike K., Lee S., Yin S., Kiyonaka S., Hamachi I. Affinity-guided oxime chemistry for selective protein acylation in live tissue systems. J. Am. Chem. Soc., 139, 14181-14191 (2017).
  11. Yamaura K., Kiyonaka S., Hamachi I. Construction of protein-based biosensors using ligand-directed chemistry for detecting analyte binding. Methods Enzymol., 589, 253-280 (2017).
  12. Wakayama S., Kiyonaka S., Arai I., Kakegawa W., Matsuda S., Ibata K., Nemoto L.Y., Kusumi A., Yuzaki M., Hamachi I. Chemical labeling for visualizing native AMPA receptors in live neurons. Nature Commun., 8, 14850 (2017).
  13. Miki T., Awa M., Nishikawa Y., Kiyonaka S., Wakabayashi M., Ishihama Y., Hamachi I. A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nature Methods, 13, 931-937 (2016).
  14. Kiyonaka S., Kubota R., Michibata Y., Sakakura M., Takahashi H., Numata T., Inoue R., Yuzaki M., Hamachi I. Allosteric activation of membrane-bound glutamate receptors using coordination chemistry within living cells. Nature Chem., 8, 958-967 (2016).
  15. Yamaura K., Kiyonaka S., Numata T., Inoue R., Hamachi I. Discovery of allosteric modulators for GABAA receptors by ligand-directed chemistry. Nature Chem. Biol., 12, 822-830 (2016).
  16. Yasueda Y., Tamura T., Fujisawa A., Kuwata K., Tsukiji S., Kiyonaka S., Hamachi I. A set of organelle-localizable reactive molecules for mitochondrial chemical proteomics in living cells and brain tissues. J. Am. Chem. Soc., 138, 7592-7602 (2016).
  17. Sawamura S., Hatano M., Takada Y., Hino K., Kawamura T., Tanikawa J., Nakagawa H., Hase H., Nakao A., Hirano M., Rotrattanadumrong R., Kiyonaka S., Mori M.X., Nishida M., Hu Y., Inoue R., Nagata R., Mori Y. Screening of Transient Receptor Potential Canonical Channel Activators Identifies Novel Neurotrophic Piperazine Compounds. Mol. Pharmacol., 89, 348-363 (2016).
  18. Nagamori S., Wiriyasermkul P., Okuda S., Kojima N., Hari Y., Kiyonaka S., Mori Y., Tominaga H., Ohgaki R., Kanai Y. Structure-activity relations of leucine derivatives reveal critical moieties for cellular uptake and activation of mTORC1-mediated signaling. Amino Acids, 48,1048-1058 (2016).
  19. Takada Y., Hirano M., Kiyonaka S., Ueda Y., Yamaguchi K., Nakahara K., Mori M.X., Mori Y. Rab3 interacting molecule 3 mutations associated with autism alter regulation of voltage-dependent Ca2+ channels. Cell Calcium, 58, 296-306 (2015).
  20. Nakao A., Miki T., Shimono K., Oka H., Numata T., Kiyonaka S., Matsushita K., Ogura H., Niidome T., Noebels J.L., Wakamori M., Imoto K., Mori Y. Compromised maturation of GABAergic inhibition underlies abnormal network activity in the hippocampus of epileptic Ca2+ channel mutant mice, tottering. Pflugers Arch., 467, 737-752 (2015).
  21. Takahashi N., Hamada-Nakahara S., Itoh Y., Takemura K., Shimada A., Ueda Y., Kitamata M., Matsuoka R., Hanawa-Suetsugu K., Senju Y., Mori M.X., Kiyonaka S., Kohda D., Kitao A., Mori Y., Suetsugu S. TRPV4 channel activity is modulated by direct interaction of the ankyrin domain to PI(4,5)P2. Nature Commun., 25, 4994 (2014).
  22. Yamada Y., Kinoshita H., Kuwahara K., Nakagawa Y., Kuwabara Y., Minami T., Yamada C., Shibata J., Nakao K., Cho K., Arai Y., Yasuno S., Nishikimi T., Ueshima K., Kamakura S., Nishida M., Kiyonaka S., Mori Y., Kimura T., Kangawa K., Nakao K. Inhibition of N-type Ca2+ channels ameliorates an imbalance in cardiac autonomic nerve activity and prevents lethal arrhythmias in mice with heart failure. Cardiovasc. Res., 104, 183-193 (2014).
  23. Yamaura K., Kuwata K., Tamura T., Kioi Y., Takaoka Y., Kiyonaka S., Hamachi I. Live cell off-target identification of lapatinib using ligand-directed tosyl chemistry. Chem. Commun., 50, 14097-14100 (2014).
  24. Miki T., Fujishima S., Komatsu K., Kuwata K., Kiyonaka S., Hamachi I. LDAI-based chemical labeling of intact membrane proteins and its pulse-chase analysis under live cell conditions. Chem. Biol., 21, 1013-1022 (2014).
  25. Kozai D., Kabasawa Y., Ebert M., Kiyonaka S., Firman, Otani Y., Numata T., Takahashi N., Mori Y., Ohwada T. Transnitrosylation directs TRPA1 selectivity in N-nitrosamine activators. Mol. Pharmacol., 85, 175-185 (2014).
  26. Kiyonaka S., Kajimoto T., Sakaguchi R., Shinmi D., Omatsu-Kanbe M., Matsuura H., Imamura H., Yoshizaki T., Hamachi I., Morii T., Mori Y. Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells. Nature Methods, 10, 1232-1238 (2013).
  27. Shi J., Geshi N., Takahashi S., Kiyonaka S., Ichikawa J., Hu Y., Mori Y., Ito Y., Inoue R. Molecular determinants for cardiovascular TRPC6 channel regulation by Ca2+/calmodulin-dependent kinase II. J. Physiol., 591, 2851-2866 (2013).
  28. Ronjat M., Kiyonaka S., Barbado M., De Waard M., Mori Y. Nuclear life of the voltage-gated Cacnb4 subunit and its role in gene transcription regulation. Channels, 7, 119-225 (2013).
  29. Ochi R., Kurotani K., Ikeda M., Kiyonaka S., Hamachi I. Supramolecular hydrogels based on bola-amphiphilic glycolipids showing color change in response to glycosidases. Chem. Commun., 49, 2115-2117 (2013).
  30. Tadmouri A., Kiyonaka S., Barbado M., Rousset M., Fablet K., Sawamura S., Bahembera E., Pernet-Gallay K., Arnoult C., Miki T., Sadoul K., Gory-Faure S., Lambrecht C., Lesage F., Akiyama S., Khochbin S., Baulande S., Janssens V., Andrieux A., Dolmetsch R., Ronjat M., Mori Y., De Waard M. Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy. EMBO J., 31, 3730-3744 (2012).
  31. Kiyonaka S., Nakajima H., Takada Y., Hida Y., Yoshioka T., Hagiwara A., Kitajima I., Mori Y., Ohtsuka T. Physical and functional interaction of the active zone protein CAST/ERC2 and the β-subunit of the voltage-dependent Ca2+ channel. J. Biochem., 152, 149-159 (2012).
  32. Nakata M., Liew F.F., Uwatoko C., Kiyonaka S., Mori Y., Katsuda Y., Endo M., Sugiyama H., Morii M. Zinc-finger proteins for site-specific protein positioning on DNA origami structures. Angew. Chem. Int. Ed., 51, 2421-2424 (2012).
  33. Katano M., Numata T., Aguan K., Hara Y., Kiyonaka S., Yamamoto S., Miki T., Sawamura S., Suzuki T., Yamakawa K., Mori Y. The juvenile myoclonic epilepsy-related protein EFHC1 interacts with the redox-sensitive TRPM2 channel linked to cell death. Cell Calcium, 51, 179-185 (2012).
  34. Takahashi N., Kuwaki T., Kiyonaka S., Numata T., Kozai D., Mizuno Y., Yamamoto S., Naito S., Knevels E., Carmeliet P., Oga T., Kaneko S., Suga S., Nokami T., Yoshida J., Mori Y. TRPA1 underlies a sensing mechanism for O2. Nature Chem. Biol., 7, 701-711 (2011).
  35. Wang H., Koshi Y., Minato D., Nonaka H., Kiyonaka S., Mori Y., Tsukiji S., Hamachi I. Chemical cell-surface receptor engineering using affinity-guided, multivalent organocatalysts. J. Am. Chem. Soc., 133, 12220-12228 (2011).
  36. Kitajima N., Watanabe K., Morimoto S., Sato Y., Kiyonaka S., Hoshijima M., Ikeda Y., Nakaya M., Ide T., Mori Y., Kurose H., Nishida M. TRPC3-mediated Ca2+ influx contributes to Rac1-mediated production of reactive oxygen species in MLP-deficient mouse hearts. Biochem. Biophys. Res. Commun., 409, 108-113 (2011).
  37. Kim M.S., Lee K.P., Yang D., Shin D.M., Abramowitz J., Kiyonaka S., Birnbaumer L., Mori Y., Muallem S. Genetic and pharmacologic inhibition of the Ca2+ influx channel TRPC3 protects secretory epithelia from Ca2+-dependent toxicity. Gastroenterology, 140, 2107-2115 (2011).
  38. Weiss N., Sandoval A., Kiyonaka S., Felix R., Mori Y., De Waard M. Rim1 modulate direct G-protein regulation of Cav2.2 channels. Pflugers Arch., 461, 447-459 (2011).
  39. Uriu Y., Kiyonaka S., Miki T., Yagi M., Akiyama S., Mori E., Nakao A., Beedle A.M., Campbell K.P., Wakamori M., Mori Y. RIMγ isoforms lacking the Rab3-binding domain induce long-lasting currents but block neurotransmitter vesicle-anchoring in voltage-dependent P/Q-type Ca2+ channels. J. Biol. Chem., 285, 21750-21767 (2010).
  40. Kinoshita H., Kuwahara K., Nishida M., Jiang Z., Rong X., Kiyonaka S., Kuwabara Y., Kurose H., Inoue R., Mori Y., Li Y., Nakagawa Y., Usami S., Fujiwara M., Yamada Y., Minami T., Ueshima K., Nakao K. Inhibition of TRPC6 channel activity contributes to the antihypertrophic effects of natriuretic peptides-guanylyl cyclase-A signaling in the heart. Circ. Res., 106, 1849-1860 (2010).
  41. Sakaguchi R., Tainaka K., Shimada N., Nakano S., Inoue M., Kiyonaka S., Mori Y., Morii T. An in vivo fluorescent sensor reveals intracellular Ins(1,3,4,5)P4 dynamics in single cells. Angew. Chem. Int. Ed., 49, 2150-2153 (2010).
  42. Numaga T., Nishida M., Kiyonaka S., Kato K., Katano M., Mori E., Kurosaki T., Inoue R., Hikida M., Putney Jr J.W., Mori Y. Ca2+ influx and protein scaffolding via TRPC3 sustain PKCβ and ERK activation in B cells. J. Cell Sci., 123, 927-938 (2010).
  43. Watanabe H., Yamashita T., Saitoh N., Kiyonaka S., Iwamatsu A., Campbell K.P., Mori Y., Takahashi T. Involvement of Ca2+ channel synprint site in synaptic vesicle endocytosis. J. Neurosci., 30, 655-660 (2010).
  44. Miyagi K., Kiyonaka S., Yamada K., Miki T., Mori E., Kato K., Numata T., Sawaguchi Y., Numaga T., Kimura T., Kanai Y., Kawano M., Wakamori M., Nomura H., Koni I., Yamagishi M., Mori Y. A pathogenic C-terminus-truncated polycystin-2 mutant enhances receptor-activated Ca2+ entry via association with TRPC3 and TRPC7. J. Biol. Chem., 284, 34400-34412 (2009).
  45. Kato K., Kiyonaka S., Sawaguchi Y., Tohnishi M., Masaki T., Yasokawa N., Mizuno Y., Mori E., Inoue K., Hamachi I., Takeshima H., Mori Y. Molecular characterization of flubendiamide sensitivity in lepidopterous ryanodine receptor Ca2+ release channel. Biochemistry, 48, 10342-10352 (2009).
  46. Sakaguchi R., Endo T., Yamamoto S., Tainaka K., Sugimoto K., Fujieda N., Kiyonaka S., Mori Y., Morii T. A single circularly permuted GFP sensor for inositol-1,3,4,5-tetrakisphosphate based on a split PH domain. Bioorg. Med. Chem., 17, 7381-7386 (2009).
  47. Sun H.S., Jackson M.F., Martin L.J., Jansen K., Teves L., Cui H., Kiyonaka S., Mori Y., Jones M., Forder J.P., Golde T.E., Orser B.A., Macdonald J.F., Tymianski M. Suppression of hippocampal TRPM7 protein prevents delayed neuronal death in brain ischemia. Nature Neurosci., 12, 1300-1307 (2009).
  48. Maruyama Y., Ogura T., Mio K., Kato K., Kaneko T., Kiyonaka S., Mori Y., Sato C. Tetrameric Orai1 is a teardrop-shaped molecule with a long, tapered cytoplasmic domain. J. Biol. Chem., 284, 13676-13685 (2009).
  49. Kiyonaka S., Kato K., Nishida M., Mio K., Numaga T., Sawaguchi Y., Yoshida T., Wakamori M., Mori E., Numata T., Ishii M., Takemoto H., Ojida A., Watanabe K., Uemura A., Kurose H., Morii T., Kobayashi T., Sato Y., Sato C., Hamachi I., Mori Y. Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound. Proc. Natl. Acad. Sci. USA, 106, 5740-5745 (2009).
  50. Takeuchi K., Nakano Y., Kato U., Kaneda M., Aizu M., Awano W., Yonemura S., Kiyonaka S., Mori Y., Yamamoto D., Umeda M. Changes in temperature preferences and energy homeostasis in dystroglycan mutants. Science, 323, 1740-1743 (2009).
  51. Olah M.E., Jackson M.F., Li H., Perez Y., Sun H.S., Kiyonaka S., Mori Y., Tymianski M., Macdonald J.F. Ca2+-dependent induction of TRPM2 currents in hippocampal neurons. J. Physiol., 587, 965-979 (2009).
  52. Ohmori I., Ouchida M., Miki T., Mimaki N., Kiyonaka S., Nishiki T., Tomizawa K., Mori Y., Matsui H. A CACNB4 mutation shows that altered Cav2.1 function may be a genetic modifier of severe myoclonic epilepsy in infancy. Neurobiol. Dis., 32, 349-354 (2008).
  53. Takahashi N., Mizuno Y., Kozai D., Yamamoto S., Kiyonaka S., Shibata T., Uchida K., Mori Y. Molecular characterization of TRPA1 channel activation by cysteine-reactive inflammatory mediators. Channels, 2, 287-298 (2008).
  54. Yamamoto S., Shimizu S., Kiyonaka S., Takahashi N., Wajima T., Hara Y., Negoro T., Hiroi T., Kiuchi Y., Okada T., Kaneko S., Lange I., Fleig A., Penner R., Nishi M., Takeshima H., Mori Y. TRPM2-mediated Ca2+ influx induces chemokine production in monocytes that aggravates inflammatory neutrophil infiltration. Nature Med., 14, 738-747 (2008).
  55. Maruyama Y., Ogura T., Mio K., Kiyonaka S., Kato K., Mori Y., Sato C. Three-dimensional reconstruction using transmission electron microscopy reveals a swollen, bell-shaped structure of transient receptor potential melastatin type 2 cation channel. J. Biol. Chem., 282, 36961-36970 (2007).
  56. Mio K., Ogura T., Kiyonaka S., Mori Y., Sato C. Subunit dissociation of TRPC3 ion channel under high-salt condition. J. Electron Microsc., 56, 111-117 (2007).
  57. Miki T., Kiyonaka S., Uriu Y., De Waard M., Wakamori M., Beedle A.M., Campbell K.P., Mori Y. Mutation associated with an autosomal dominant cone-rod dystrophy CORD7 modifies RIM1-mediated modulation of voltage-dependent Ca2+ channels. Channels, 1 144-147 (2007).
  58. Kiyonaka S., Wakamori M., Miki T., Uriu Y., Nonaka M., Bito H., Beedle A.M., Mori E., Hara Y., De Waard M., Kanagawa M., Itakura M., Takahashi M., Campbell K.P., Mori Y. RIM1 confers sustained activity and neurotransmitter vesicle anchoring to presynaptic Ca2+ channels. Nature Neurosci., 10, 691-701 (2007).
  59. Mio K., Ogura T., Kiyonaka S., Hiroaki Y., Tanimura Y., Fujiyoshi Y., Mori Y., Sato C. The TRPC3 channel has a large internal chamber surrounded by signal sensing antennas.J. Mol. Biol., 367, 373-383 (2007).
  60. Yamazaki K., Shigetomi E., Ikeda R., Nishida M., Kiyonaka S., Mori Y., Kato F. Blocker-resistant presynaptic voltage-dependent Ca2+ channels underlying glutamate release in mice nucleus tractus solitarii. Brain Res., 1104, 103-113 (2006).
  61. Ojida A., Honda K., Shinmi D., Kiyonaka S., Mori Y., Hamachi I. Oligo-Asp tag/Zn(II) complex probe as a new pair for labeling and fluorescence imaging of proteins. J. Am. Chem. Soc., 128, 10452-10459 (2006).
  62. Tamaru S., Kiyonaka S., Hamachi I. Three distinct read-out modes for enzyme activity can operate in a semi-wet supramolecular hydrogel. Chem. Eur. J., 11, 7294-7304 (2005).
  63. Zhou S.-L., Matsumoto S., Tian H.-D., Yamane H., Ojida A., Kiyonaka S., Hamachi I. pH-responseive shrinkage/swelling of a supramolecular hydrogel composed of two small amphiphilic molecules. Chem. Eur. J., 11, 1130-1136 (2005).
  64. Aiba Y., Oh-hora M., Kiyonaka S., Kimura Y., Hikita A., Mori Y., Kurosaki T. Activation of RasGRP3 by phosphorylation of Thr-133 is required for B cell receptor-mediated Ras activation. Proc. Natl. Acad. Sci. USA, 101,16612-16617 (2004).
  65. Kiyonaka S., Sada K., Yoshimura I., Shinkai S., Kato N., Hamachi I. Semi-wet peptide/protein array using supramolecular hydrogel. Nature Mater., 3, 58-64 (2004).
  66. Kiyonaka S., Zhou S.-L., Hamachi I. pH-responsive phase transition of supramolecular hydrogel consisting of glycosylated amino acetate and carboxylic acid derivative. Supramolecular Chem., 15, 521-528 (2003).
  67. Kiyonaka S., Shinkai S., Hamachi I. Combinatorial library of low molecular-weight organo- and hydrogelators based on glycosylated aminoacid derivatives by solid-phase synthesis. Chem. Eur. J., 9, 976-984 (2003).
  68. Kiyonaka S., Sugiyasu K., Shinkai S., Hamachi I. First thermally responsive supramolecular polymer based on glycosylated amino acid. J. Am. Chem. Soc., 124, 10954-10955 (2002).
  69. Bewley C.A., Kiyonaka S., Hamachi I. Site-specific discrimination by cyanovirin-N for α-linked trisaccharides comprising the three arms of man8 and man9. J. Mol. Biol., 322, 881-889 (2002).
  70. Hamachi I., Kiyonaka S., Shinkai S. Solid-phase lipid synthesis (SPLS)-2: incidental discovery of organogelators based on artificial glycolipids. Tetrahedron Lett., 42, 6141-6145 (2001).
  71. Hamachi I., Kasagi N., Kiyonaka S., Nagase T., Mito-oka Y., Shinkai S. Pd(en) as a sequence-selective molecular pinch for α-helical peptides. Chem. Lett., 16-17 (2001).
  72. Murakami H., Okusa Y., Kiyonaka S., Hamachi I., Shinkai S., Nakashima N. The synthesis of a reconstituted C60-modified protein. Chem. Lett., 46-47 (2000).
  73. Hamachi I., Kiyonaka S., Shinkai S. Solid-phase lipid synthesis (SPLS) for construction of an artificial glycolipid library. Chem. Commun., 1281-1282 (2000).
  74. Inoue K., Ono Y., Kanekiyo Y., Kiyonaka S., Hamachi I., Shinkai S. Facile preparation of robust organic gels by cross-link of a sugar-integrated getator by toluene-2,4-diisocyanate. Chem. Lett., 255-256 (1999).

英文総説・論評 (English review or commentary)

    　
  1. Miura Y., Senoo A., Doura T., Kiyonaka S.　Chemogenetics of cell surface receptors: beyond genetic and pharmacological approaches.RSC Chem. Biol.,3, 269-287 (2022). DOI: 10.1039/d1cb00195g.
  2. Tsai Y.H., Doura T., Kiyonaka S.Tethering-based chemogenetic approaches for the modulation of protein function in live cells. Chem. Soc. Rev., 50, 7909-7923 (2021). DOI: 10.1039/d1cs00059d.
  3. Sakamoto S, Kiyonaka S, Hamachi I. Construction of ligand assay systems by protein-based semisynthetic biosensors. Curr. Opin. Chem. Biol., 50, 10-18 (2019).
  4. Okabe K., Sakaguchi R., Shi B., Kiyonaka S. Intracellular thermometry with fluorescent sensors for thermal biology. Pflugers Arch., 470, 717-731 (2018).
  5. Mori Y., Takahashi N., Kurokawa T., Kiyonaka S. TRP channels in oxygen physiology: distinctive functional properties and roles of TRPA1 in O2 sensing. Proc. Jpn. Acad. Ser. B Phys. Biol. Sci., 93, 464-482 (2017).
  6. Kiyonaka S., Sakaguchi R., Hamachi I., Morii T., Yoshizaki T., Mori Y. Validating subcellular thermal changes revealed by fluorescent thermosensors. Nature Methods, 12, 801-802 (2015).
  7. Sakaguchi R., Kiyonaka S., Mori Y. Fluorescent sensors reveal subcellular thermal changes. Curr. Opin. Biotechnol., 31, 57-64 (2015).
  8. Mori Y., Kiyonaka S., Kanai Y. Transportsomes and channelsomes: are they functional units for physiological responses? Channels, 5, 387-390 (2011).
  9. Numata T., Kiyonaka S., Kato K., Takahashi N., Mori Y. Activation of TRP channels in mammalian systems. TRP Channels, Taylor & Francis, 43-90 (2011).
  10. Mori Y., Kajimoto T., Nakao A., Takahashi N., Kiyonaka S. Receptor signaling integration by TRP channelsomes. Transient Receptor Potential Channels, Springer, 373-389 (2011).

和文総説

  1. 三浦 裕太, 小島 憲人, 清中 茂樹, 配位ケモジェネティクスによるグルタミン酸受容体の活性制御, 日薬理誌, 157, 366-370 (2022)
  2. 杉原 佑太朗, 小島 憲人, 清中 茂樹, リガンド指向性2段階ラベル化によるAMPA型グルタミン酸受容体の精密動態解析, 日薬理誌, 157, 191-195 (2022)
  3. 曽我 恭平, 清中 茂樹, 迅速ケミカルラベル化法によるAMPA型グルタミン酸受容体の精密動態解析, 生化学, 94, 292-297 (2022)
  4. 妹尾 暁暢, 清中 茂樹, 配位ケモジェネティクスによる代謝型グルタミン酸受容体の制御,化学と工業, 75,115 (2022)
  5. 坂口 怜子, 金岡 英徳, 清中 茂樹, タンパク質温度センサーを利用した細胞内温度計測 熱測定, 48, 152-158 (2021)
  6. 三浦 裕太, 清中 茂樹, GPCRおよびGタンパク質シグナルを特異的に制御するケモジェネティクス法, 細胞, 53, 328-330 (2021).
  7. 小島 憲人, 清中 茂樹, 浜地 格, ケモジェネティクスによる神経伝達物質受容体の人為的な活性制御, 医学のあゆみ, 262, 11, 1036-1037 (2017).
  8. 清中 茂樹, 小島 憲人, 浜地 格, ケモジェネティクス－遺伝子工学と化学的なアプローチを組み合わせた受容体の人為的な活性制御法－, Clinical Neuroscience, 35, 260-261 (2017).
  9. 清中 茂樹, 坂口 怜子, 森 泰生, 吉崎 武尚, 細胞内温度を測定できるか－論争を整理する, 生体の科学, 67, 363-368 (2016).
  10. 坂口怜子, 清中茂樹, 森泰生, 細胞内熱産生機構の可視化, 医学のあゆみ, 252, 256-257 (2015).
  11. 清中 茂樹, 坂口 怜子, 蛍光性タンパク質サーモセンサーによる細胞内温度変化の可視化, 生体の科学, 66, 169-174 (2015).
  12. 清中 茂樹, 坂口 怜子, 森 泰生, 蛍光性センサータンパク質による細胞内温度分布の可視化, 生物物理, 54, 253-256 (2014).
  13. 清中 茂樹, 高橋 重成, 森 泰生, 活性酸素で活性化されるTRPチャネル, ファルマシア, 48, 31-36 (2012).
  14. 瓜生 幸嗣, 清中 茂樹, 高田 宜則, 森 泰生, Ca2+チャネルソーム, 遺伝子医学MOOK, 36, 102-105 (2011).
  15. 清中 茂樹, 加藤 賢太, 西田 基宏, 森 泰生, TRPCチャネルを標的とした新規Ca2+拮抗薬の創製, 薬学雑誌, 130, 303-311 (2010).
  16. 清中 茂樹, 高橋 重成, 香西 大輔, 森 泰生, 化学物質の感覚分子生物学－TRPチャネルはどのように物質を感知し脳へ伝えるか, 化学, 64, 27-33 (2009).
  17. 山本 伸一郎, 高橋 重成, 清中 茂樹, 森 泰生, 活性化学種感応のチャネル機構の探求, 蛋白質核酸酵素, 54, 212-223 (2008).
  18. 清中 茂樹, 三木 崇史, 瓜生 幸嗣, 森 泰生, 神経伝達物質放出におけるCa2+チャネル複合体形成の生理的意義, 生化学, 80, 658-661 (2008).
  19. 清中 茂樹, 加藤 賢太, 高橋 重成, 正木 隆男, 森 泰生, カルシウムシグナル制御チャネルの作用物と創薬, 医学のあゆみ, 223, 1136-1137 (2007).
  20. 加藤 賢太, 清中 茂樹, 森 泰生, CRACチャネル分子実体Orai1, 医学のあゆみ, 221, 601-602 (2007).
  21. 清中 茂樹, 森 泰生, 新規カルシウム拮抗薬標的としてのTRPチャネル, 日薬雑誌, 126, 317-320 (2005).
  22. 清中 茂樹, 森 泰生, TRPチャネル－細胞内外の環境感知センサー－, 生体の科学, 55, 524-525 (2004).
  23. 吉村 息吹, 清中 茂樹, 浜地 格, 超分子ヒドロゲルを利用したセミウェット型ペプチド／プロテインアレイの開発, Bioベンチャー, 5-6, 42-43 (2004).
  24. 清中 茂樹, 周 善来, 浜地 格, 多様な化合物郡の中からナノバイオ材料を創る, 未来材料, 3, 36-45 (2003).
  25. 浜地 格, 清中 茂樹, 新しい生体材料の設計へのアプローチ, 生体材料, 18, 142-145 (2001).
  26. 清中 茂樹, 浜地 格, 新海 征治, モジュール法を用いる糖鎖誘導体の合成とその分子集合超構造, 表面, 39, 206-214 (2001).