Watanabe Lab. | Nagoya University

Welcome to Watanabe Labようこそ

Introduction研究室の紹介

Our life is supported by biomacromolecules such as protein, which exhibit various functions. The three-dimensional structural information of these biomacromolecules is not only indispensable for understanding life phenomena, but also for applications such as drug discovery and development of new functional proteins.

Our laboratory is focused on the structural analysis method using X-ray diffraction, such as protein crystallography and small-angle scattering, to elucidate the relationship between structure and function of various proteins. In addition to applied research, we are also working on new methodological developments of structural research, such as beamline technologies of synchrotron radiation, the experimental equipment capable of diffraction measurement under high pressure (1 GPa, 10,000 atm) environment, or combined analysis with computer simulation.

We are pursuing basic research on the protein, feeling the wonder and fun of the protein structure. We are aiming to create the novel proteins with functions of high efficiency, pressure resistance, heat resistance, and so on, in the future.

What's New新着情報

2018.11: "Structural basis of chimpanzee APOBEC3H dimerization stabilized by double-stranded RNA" was published.
Matsuoka, T. et al., Nucl. Acids Res., 46, 10368-10379 (2018).

2018.9: "High-pressure protein crystal structure analysis of Escherichia coli dihydrofolate reductase complexed with folate and NADP⁺" was published.
Nagae, T. et al., Acta Cryst. D, 74, 895-905 (2018).

2018.4: "Similar structural stabilities of 3-isopropylmalate dehydrogenases from the obligatory piezophilic bacterium Shewanella benthica strain DB21MT-2 and its atmospheric congener S. oneidensis strain MR-1" was published.
E. Ohmae et al., BBA - Proteins Proteomics, 1866, 680-691 (2018).

2017.4: 渡邉信久，山田裕之，永江峰幸, 高圧力学会誌(高圧力の科学と技術)，27(1), 18-25 (2017) was published (in Japanese).

2017.3: "Mapping Region of Human Restriction Factor APOBEC3H Critical for Interaction with HIV-1 Vif" was published.
M. Nakashima et al., J. Mol. Biol., 429(8), 1262-1276 (2017).

2016.2: Press release about the result of the joint research between Rikkyo University, JAMSTEC, Hiroshima University and our laboratory was press released (in Japanese)．

2016.1: "Unique Flap Conformation in an HIV-1 Protease with High-Level Darunavir Resistance" by M. Nakashima et al. was published.

2015.10: "Structural Insights into HIV-1 Vif-APOBEC3F Interaction" by M. Nakashima et al. was publishded.

2015.9: High Pressure Macromolecular Crystallography (High Pressures Bioscience, Eds. K. Asakura and H. Matsuki, Springer Netherlands, 2015) by Prof. N. Watanabe was published．

2015.5: Official use were started at BL2S1 Nagoya University beamline for protein crystallography in Aichi SR.

2015.2: "High-pressure protein crystallography of hen egg-white lysozyme" by H. Yamada et al. was published.

2015.2: "Analysis of the HindIII-catalyzed reaction by time-resolved crystallography" was published.