メンバー紹介

名前：　村山　恵司　（むらやま　けいじ）
生年月日：　1987年7月
メールアドレス：　murayama(at)chembio.nagoya-u.ac.jp
（アドレスご利用の際は(at)を@に変えてください）

研究内容

  1. 新規人工核酸XNAの開発
  2. 人工核酸を利用した高機能ツールの開発
  3. 化学ライゲーションを利用した人工生命・原始生命に関する研究

プロフィール

2006.3 三重県立四日市高等学校卒業
2010.3 名古屋大学工学部化学・生物工学科卒業
2012.3 名古屋大学大学院工学研究科物質制御工学専攻博士前期課程修了
2012.4 名古屋大学大学院工学研究科物質制御工学専攻博士後期課程入学
2012.4 日本学術振興会特別研究員 (DC1)
2015.3 名古屋大学大学院工学研究科物質制御工学専攻博士後期課程修了
博士(工学)

職歴

2015.4-2016.1 ベンチャー・ビジネス・ラボラトリー(VBL) 中核的研究機関研究員
2016.2- 名古屋大学大学院工学研究科 助教
2020.1- （兼務）名古屋大学 未来社会創造機構 ナノライフシステム研究所 助教
2023.4- （兼務）JST創発的研究支援事業 創発研究者(福島パネル)

主な業績

論文[査読あり]

  1. "A Light-powered single-stranded DNA molecular motor with colour-selective single-step control"
    Anderson, T.; Wu, W.; Sirbu, O.; Tong, K.; Winna, S.; Liu, X. R.; Kou, B.; Murayama, K.; Asanuma, H.; Wang, Z.
    Angew. Chem. Int. Ed., 2024, 63, e202405250. DOI: doi.org/10.1002/anie.202405250
    Selected as a "Hot paper"
  2. "DNA reaction system that acquires classical conditioning"
    Nakakuki, T.; Toyonari, M.; Aso, K.; Murayama, K.; Asanuma, H.; de Greef, T.
    ACS Synth. Biol., 2024, 13, 521-529. DOI: https://doi.org/10.1021/acssynbio.3c00459
  3. "Rapid Chemical Ligation of DNA and Acyclic Threoninol Nucleic Acid (aTNA) for Effective Nonenzymatic Primer Extension "
    Okita, H.; Kondo, S.; Murayama, K.; Asanuma, H.
    J. Am. Chem. Soc., 2023, 145, 17872-17880. DOI: 10.1021/jacs.3c04979
    Selected as a "Supplementary Cover"
  4. "DNA Concentration Regulator That can be Driven for a Long Time"
    Nakakuki, T.; Murayama, K.; Asanuma, H.
    New Gener. Comput., 2022, 40, 681-702. DOI: https://doi.org/10.1007/s00354-022-00173-3
  5. "Kinesin motors driven microtubule swarming triggered by UV light"
    Ishii, S.; Akter, M.; Murayama, K.; Kabir, A. M. R.; Asanuma, H.; Sada, K.; Kakugo, A.
    Polym. J., 2022, 54, 1501-1507. DOI: https://doi.org/10.1038/s41428-022-00693-1
  6. "Methyl group configuration on acyclic threoninol nucleic acids (aTNAs) impacts supramolecular properties"
    Murayama, K.; Kashida, H.; Asanuma, H.
    Org. Biomol. Chem., 2022, 20, 4115-4122. DOI: 10.1039/d2ob00266c
    Selected as a "Front Cover"
  7. "Orthogonal amplification circuits composed of acyclic nucleic acids enable RNA detection"
    Chen, Y.; Nagao, R.; Murayama, K.; Asanuma, H.
    J. Am. Chem. Soc., 2022, 144, 5887-5892. DOI: 10.1021/jacs.1c12659
    Selected as a "Supplementary Cover"
  8. "Renewable DNA proportional-integral controller with photoresponsive molecules"
    Tamba, M.; Murayama, K.; Asanuma, H.; Nakakuki, T.
    Micromachines, 2022, 13, 193. doi.org/10.3390/mi13020193
  9. "Signal Amplification Circuit Composed of Serinol Nucleic Acid for RNA Detection"
    Chen, Y.; Murayama, K.; Asanuma, H.
    Chem. Lett., 2022, 51, 330-333. DOI:10.1246/cl.210813
  10. "Unexpected Dissociation of Photoresponsive UV-ON DNA Carrying p-tert-Butyl Azobenzene under UV Light Irradiation"
    Ishii, S.†; Murayama, K.†; Sada, K.; Asanuma, H.; Kakugo, A.
    Chem. Lett., 2022, 51, 292-295. DOI:10.1246/cl.210788
    (†; These authors contributed equally to this work.)
  11. "Pyrene modified serinol nucleic acid nanostructure converts chirality of threoninol nucleic acids into circularly polarized luminescence signals"
    Kashida, H.; Nishikawa, K.; Ito Y.; Murayama, K.; Hayashi, I.; Kakuta, T.; Ogoshi, T.; Asanuma, H.
    Chem. Eur. J., 2021, 27, 14582-14585. DOI: 10.1002/chem.202102333
    Selected as a "Cover Feature"
  12. "Light-regulated liquid-liquid phase separation for spatiotemporal protein recruitment and cell aggregation"
    Ikeuchi, N.; Komachi, T.; Murayama, K.; Asanuma, H.; Maruyama, A.; Shimada, N.
    ACS Appl. Mater. Interfaces, 2021, 13, 5652-5659. DOI: 10.1021/acsami.0c22314
  13. "Nonenzymatic polymerase-like template-directed synthesis of acyclic L-threoninol nucleic acid"
    Murayama, K.; Okita, H.; Kuriki, T.; Asanuma, H.
    Nat. Commun., 2021, 12, 804. DOI: 10.1038s41467-021-21128-0
    Selected as s featured article
  14. "Dual crosslinking photo-switches for orthogonal photo-control of hybridization between serinol nucleic acid and RNA"
    Yamano, Y.; Murayama, K.; Asanuma, H.
    Chem. Eur. J., 2021, 27, 4599-4604. doi.org/10.1002/chem.202003528
    Selected as a "Cover Feature"
  15. "Intrastrand backbone-nucleobase interactions stabilize unwound right-handed helical structures of heteroduplexes of L-aTNA/RNA and SNA/RNA"
    Kamiya, Y.; Satoh, T.; Kodama, A.; Suzuki, T.; Murayama, K.; Kashida, H.; Uchiyama, S.; Kato, K.; Asanuma, H.
    Commun. Chem., 2020, 3, 156. DOI: 10.1038/s42004-020-00400-2
  16. "Photo-regulated trajectories of gliding microtubules conjugated with DNA"
    Akter, M.; Keya, J.J.; Kabir, A.M.R.; Asanuma, H.; Murayama, K.; Sada, K.; Kakugo, A.
    Chem. Commun., 2020, 56, 7953-7956. DOI: 10.1039/d0cc03124k
  17. "A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity"
    Chen, Y.; Murayama, K.; Kashida, H.; Kamiya, Y.; Asanuma, H.
    Chem. Commun., 2020, 56, 5358-5361. DOI: 10.1039/D0CC01865A
    Selected as a "Back cover"
  18. "Incorporation of Pseudo-complementary Bases 2,6-Diaminopurine and 2-Thiouracil into Serinol Nucleic Acid (SNA) to Promote SNA/RNA Hybridization"
    Kamiya, Y.; Sato, F.; Murayama, K.; Kodama, A.; Uchiyama, S.; Asanuma, H.
    Chem. Asian J., 2020, 15, 1266-1271. DOI: 10.1002/asia.201901728
    Selected as a "Cover Picture"
  19. "A Quencher‐Free Linear Probe from Serinol Nucleic Acid with a Fluorescent Uracil Analogue"
    Murayama, K.; Asanuma, H.
    ChemBioChem, 2020, 21, 120-128. DOI: 10.1002/cbic.201900498
    Selected as a "Cover Feature"
  20. "8-Pyrenylvinyl Adenine Controls Reversible Duplex Formation between Serinol Nucleic Acid and RNA by [2+2] Photocycloaddition"
    Murayama, K.; Yamano, Y.; Asanuma, H.
    J. Am. Chem. Soc., 2019, 141, 9485-9489. DOI: 10.1021/jacs.9b03267
    Selected as a "Supplementary Cover"
  21. "cis-on/trans-off of DNA hybridization with alkylthio-azobenzene on L-threoninol responding to visible light"
    Asanuma, H.; Ishikawa, T.; Yamano, Y.; Murayama, K.; Liang, X.
    ChemPhotoChem, 2019, 3, 418-424. DOI: 10.1002/cptc.201900060
    Selected as a "VIP"
    Selected as a "Cover Feature"
  22. "Orthogonally Photocontrolled Non-Autonomous DNA Walker"
    Skugor, M.; Valero, J.; Murayama, K.; Centola, M.; Asanuma, H.; Famulok, M.
    Angew. Chem. Int. Ed., 2019, 58, 6948-6951. DOI: 10.1002/anie.201901272
  23. "The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons"
    Murayama, K.; Kashida H.; Asanuma, H.
    Method in Molecular Biology, 2019, Vol. 1973, : Non-Natural Nucleic Acids.
  24. "Introduction of 2,6-diaminopurines into serinol nucleic acid (SNA) improves anti-miRNA performance"
    Kamiya, Y.; Donoshita, Y.; Kamimoto, H.; Murayama, K.; Ariyoshi, J.; Asanuma, H.
    ChemBioChem, 2017, 18, 1917-1922.
  25. "Antisense oligonucleotide modified with serinol nucleic acid (SNA) induces exon skipping in mdx myotubes"
    Le, B. T.; Murayama, K.; Shabanpoor, F.; Asanuma, H.; Veedu, R. N.
    RSC Advances, 2017, 7, 34049-34052.
  26. "D-aTNA circuit orthogonal to DNA can be operated by RNA input via SNA"
    Murayama, K.; Nagao, R.; Asanuma, H.
    ChemistrySelect, 2017, 2(20), 5624-5627.
  27. "Effect of Methyl Group on Acyclic Serinol Scaffold for Tethering Dyes on DNA Duplex stability"
    Murayama, K.; Asanuma, H.
    ChemBioChem, 2017, 18, 142-149.
  28. "Strand-invading linear probe combined with unmodified PNA"
    Aasanuma, H.; Niwa, R.; Akahane, M.; Murayama, K.; Kashida, H.; Kamiya, Y.
    Bioorg. Med. Chem., 2016, 24, 4129-4137. DOI:10.1016/j.bmc.2016.06.055
  29. "Visible Light-Triggered Crosslinking of DNA Duplex by Reversible [2+2] Photocycloaddition of Styrylpyrene"
    Doi, T.; Kawai, H.; Murayama, K.; Kashida, H.; Asanuma, H.
    Chem. Eur. J., 2016, 22, 10533-10538
  30. "Azobenzene C-nucleosides for photo-controlled hybridization of DNA at room temperature"
    Goldau, T.; Murayama, K.; Brieke, C.; Asanuma, H.; Heckel, A.
    Chem. Eur. J., 2015, 21, 17870-17876, DOI : 10.1002/chem.201503303
  31. "Ultra-Sensitive Molecular Beacon Designed with Totally Serinol Nucleic Acid (SNA) for Monitoring mRNA in Cell"
    Murayama, K.; Kamiya, Y.; Kashida, H.; Asanuma, H.
    ChemBioChem, 2015, 16, 1298-1301, DOI: 10.1002/cbic.201500167
  32. "Acyclic L-Threoninol Nucleic Acid (L-aTNA) with Suitable Structural Rigidity Cross-pairs with DNA and RNA"
    Murayama, K.; Kashida, H.; Asanuma, H.
    Chem. Commun., 2015, 51, 6500-6503
    Selected as a "Back Cover"
  33. "Reversible photoswitching of RNA hybridization at room temperature with an azobenzene C-nucleoside."
    Goldau, T.†; Murayama, K.†; Brieke, C. Steinwand, S.; Mondal, P.; Biswas, M.; Burghardt, I.; Wachtveitl, J.; Asanuma, H.; Heckel, A.
    Chem. Eur. J., 2015, 21, 2845-2854. (†; These authors contributed equally to this work.)
  34. "Enhancement of stability and activity of siRNA by terminal substitution with Serinol Nucleic Acid (SNA)."
    Kamiya, Y.; Takai, J.; Ito, H.; Murayama, K.; Kashida, H.; Asanuma, H.
    ChemBioChem, 2014, 15, 2549-2555.
    Selected as a "Back Cover"
  35. "The “sugar-deficient” G-quadruplex: incorporation of aTNA in quadruplex structures."
    Zhou, J.†; Murayama, K.†; Amrane, S.; Kashida, H.; Bourdoncle, A.; Asanuma, H.; Mergny, J. L.
    Chem. Sci., 2013, 4, 3693-3698. (†; These authors contributed equally to this work.)
  36. "Highly stable duplex formation by artificial nucleic acids aTNA and SNA with acyclic scaffolds."
    Murayama, K.; Tanaka, Y.; Toda, T.; Kashida, H.; Asanuma, H.
    Chem. Eur. J., 2013, 19, 14151-14158.
  37. "Control of the Chirality and Helicity of Oligomers of Serinol Nucleic Acid (SNA) by Sequence Design"
    Kashida, H.; Murayama, K.; Toda, T.; Asanuma, H.
    Angew. Chem. Int. Ed., 2011, 50, 1285-1288.
  38. "Unexpectedly stable artificial duplex from flexible acyclic threoninol"
    Asanuma, H.; Toda, T.; Murayama, K.; Liang, X.G.; Kashida, H.
    J. Am. Chem. Soc., 2010, 132, 14702-14703.
　　

総説[査読あり]

  1. "Highly Functional Acyclic Xeno Nucleic Acids"
    Murayama, K.; Okita, H.; Asanuma, H.
    Bull. Chem. Soc. Jpn., 2023, 96, 1179-1187. DOI: doi.org/10.1246/bcsj.20230188
  2. "Functionalization of Acyclic Xenonucleic Acid with Modified Nucleobases"
    Murayama, K.; Yamano, Y.; Asanuma, H.
    Polym. J., 2023, 55, 743-752. DOI: doi.org/10.1038/s41428-023-00776-7
  3. "Xeno nucleic acids (XNAs) having non-ribose scaffolds with unique supramolecular properties"
    Asanuma, H.; Kamiya, Y.; Kashida, H.; Murayama, K.
    Chem. Commun., 2022, 28, 3993. DOI: 10.1039/d1cc05868a
    Selected as a "Front cover"
  4. "Design and Hybridization Properties of Acyclic Xeno Nucleic Acid Oligomers"
    Murayama, K.; Asanuma, H.
    ChemBioChem, 2021, 22, 2507-2515. doi.org/10.1002/cbic.202100184
    Selected as "Cover Feature"
  5. "The DNA duplex as an aqueous one-dimensional soft crystal scaffold for photochemistry."
    Asanuma, H.; Murayama, K.; Kamiya, Y.; Kashida, H.
    Bull. Chem. Soc. Jpn., 2018, 91, 1739-1748.
  6. "Design of photo-functional oligonucleotide by "copolymerization" of natural nucleobases with base-surrogate prepared from acyclic scaffold."
    Asanuma, H.; Murayama, K.; Kamiya, Y.; Kashida, H.
    Polymer J., 2017, 49, 279-289
  7. "Acyclic artificial nucleic acids with phosphodiester bonds exhibit unique functions"
    Kashida, H., Murayama, K., Asanuma, H.
    Polymer J., 2016, 48, 781-786

解説論文

  1. "RNA以前の世界を妄想する"
    浅沼浩之, 村山恵司
    化学と工業, 2022, 75 (8), 552-554.
  2. "RNA以前に非環状型XNAがPre-RNAとして存在したか?"
    浅沼浩之, 沖田ひかり, 村山恵司
    Viva Origino, 2021, 49, 9. DOI: https://doi.org/10.50968/vivaorigino.49_9
  3. "Nonenzymatic Polymerase-Like Primer Extension of Acyclic L-Threoninol Nucleic Acid"
    Keiji Murayama
    会誌「高分子」, 2022, 71, 6.
  4. "光応答性XNA　～アゾベンゼンに頼らない新たな光制御～"
    村山恵司
    日本核酸化学会誌, 2021, No. 5, 2-7.
  5. "光駆動型DNAナノマシン"
    浅沼浩之, 村山恵司, 神谷由紀子, 樫田　啓
    高分子, 2021, 70, 550-552.
  6. "核酸医薬の創製に向けた人工核酸の開発"
    樫田啓、村山恵司、浅沼浩之
    バイオマテリアル, 2020, 38(2), 124-129
  7. "非環状型人工核酸のケミカルライゲーション"
    村山恵司
    Newsletter-生体機能関連部会-, 2019, Vol. 34, 17-22.
  8. "天然に存在しない非環状構造の核酸アナログ-人工の非環状骨格で神の設計した環状骨格に挑む"
    村山恵司、浅沼浩之
    化学, 2016, 71(3), 66-67
  9. "新規人工核酸SNAを用いたRNAイメージング"
    樫田啓、村山恵司、浅沼浩之
    生体の科学 2015, 66(2), 145-150
    10. 　　

著書

  1. "6.5 Orthogonality of Nucleic Acids"
    Keiji Murayama, Hiroyuki Asanuma
    "Molecular Robotics An Introduction", Satoshi Murata Ed., Springer, p.230-238. (2022)
  2. "2-26　DNAポリマー"
    村山恵司、浅沼浩之
    "高分子材料の事典", 高分子学会編, 朝倉書店 (2022)
  3. "5.5 核酸の直交性"
    浅沼浩之、村山恵司
    "分子ロボティクス概論", 編集：村田 智, CBI学会出版 (2019)
  4. "非環状骨格型人工核酸：aTNA, SNA"
    神谷由紀子、村山恵司、樫田 啓、浅沼浩之
    "核酸医薬の創製と応用展開", 監修：和田 猛, p79-86, シーエムシー出版(2016)
    5. 　　

受賞歴

  • 2015年　IGER Annual Meeting 2014 Oral Presentation Award
  • 2016年　第26回バイオ・高分子シンポジウム, 若手研究者奨励講演賞
  • 2019年　第13回バイオ関連化学シンポジウム, 講演賞
  • 2019年　ISNAC 2019, 若手優秀講演賞（大塚賞）
  • 2021年　2020年度高分子研究奨励賞
  • 2023年　日本化学会　第72回進歩賞
  • 2024年　令和5年度　赤崎賞

招待講演

  1. "人工生命システム創製を目指した人工核酸XNAの鎖伸長反応の開発"
    村山恵司
    第54回中部化学関係学協会支部連合秋季大会（2023年11月11～12日、三重大学工学部）
  2. "人工生命システム構築に向けた人工核酸XNAの鎖伸長反応"
    村山恵司
    細胞を創る研究会（2023年9月25～26日、東京大学　駒場キャンパス）
  3. "Nonenzymatic Primer Extension of Acyclic L-Threoninol Nucleic Acid Using Highly Effective Chemical Ligation"
    Keiji Murayama
    ICPAC Bali 2023 (2023年9月15日、ハイブリッド)
  4. "Photoregulation of Artificial Nucleic Acid via Photo-Cycloaddition of Modified Adenine Residues for Chemical AI"
    Keiji Murayama, Yuuhei Yamano, Hiroyuki Asanuma
    第59回日本生物物理学会年会 (2021年11月25-27日、On-line)
  5. "化学AI構築に向けた光応答性人工核酸の設計と非酵素的鎖伸長反応の開発"
    村山恵司
    「細胞を創る」研究会14.0 (2021年11月4-5日、On-line)
  6. "安定な二重鎖を形成する非環状型人工核酸の設計と高機能化"
    村山恵司
    2021年度 北海道高分子若手研究会 (2021年8月27日、On-line)
  7. "非環状型人工核酸の鋳型重合による配列複製と機能化"
    村山恵司
    第70回高分子学会年次大会 (2021年5月26～28日、On-line)
  8. "人工生命の創製を目指した非環状型人工核酸の開発と鋳型特異的な鎖伸長反応への応用"
    村山恵司
    第174回東海高分子研究会講演会 (2020年8月29日、On-line)
    9. 　　

趣味

作曲/編曲/ミキシング、棚田巡り、修理