Members

Name：　Keiji Murayama
Date of Birth： Jul. 1987
E-mail：　murayama(at)chembio.nagoya-u.ac.jp

Education

2010 B.S. Nagoya University
2012 M.S. Nagoya University
2015 Ph.D. Nagoya University

Professional Experience

2015.4-2016.1 Postdoctoral Fellow, Venture Business Laboratory (VBL), Nagoya University
2016.2- Assistant Professor, Graduate School of Engineering, Nagoya University

Publication

Original Papers

  1. "Highly Effective Chemical Ligation of DNA and L-aTNA"
    Okita, H.; Murayama, K.; Asanuma, H.
    Current Protocols, 2025, accepted, . DOI:
    2. ]
  2. "Chirality-Promoted Chemical Ligation and Reverse Transcription of Acyclic Threoninol Nucleic Acid"
    Okita, H.; Murayama, K.; Asanuma, H.
    J. Am. Chem. Soc., 2025, accepted, . DOI:
  3. "Reversible Optical Control of Receptor Tyrosine Kinase Activity and ERK Dynamics Using Azobenzene-Carrying DNA Aptamer Agonist"
    Wakano, M.; Tsunoda, M.; Murayama, K.; Morimoto, J.; Ueki, R.; Aoyama-Ishiwatari, S.; Hirabayashi, Y.; Asanuma, H.; Sando, S.
    J. Am. Chem. Soc., 2025, accepted, DOI: https://doi.org/10.1021/jacs.5c01559
  4. "A Light-powered single-stranded DNA molecular motor with colour-selective single-step control"
    Anderson, T.; Wu, W.; Sirbu, O.; Tong, K.; Winna, S.; Liu, X. R.; Kou, B.; Murayama, K.; Asanuma, H.; Wang, Z.
    Angew. Chem. Int. Ed., 2024, 63, e202405250. DOI: doi.org/10.1002/anie.202405250
    Selected as a "Hot paper"
  5. "DNA reaction system that acquires classical conditioning"
    Nakakuki, T.; Toyonari, M.; Aso, K.; Murayama, K.; Asanuma, H.; de Greef, T.
    ACS Synth. Biol., 2024, 13, 521-529. DOI: https://doi.org/10.1021/acssynbio.3c00459
  6. "Rapid Chemical Ligation of DNA and Acyclic Threoninol Nucleic Acid (aTNA) for Effective Nonenzymatic Primer Extension "
    Okita, H.; Kondo, S.; Murayama, K.; Asanuma, H.
    J. Am. Chem. Soc., 2023, 145, 17872-17880. DOI: 10.1021/jacs.3c04979
    Selected as a "Supplementary Cover"
  7. "DNA Concentration Regulator That can be Driven for a Long Time"
    Nakakuki, T.; Murayama, K.; Asanuma, H.
    New Gener. Comput., 2022, 40, 681-702. DOI: https://doi.org/10.1007/s00354-022-00173-3
  8. "Kinesin motors driven microtubule swarming triggered by UV light"
    Ishii, S.; Akter, M.; Murayama, K.; Kabir, A. M. R.; Asanuma, H.; Sada, K.; Kakugo, A.
    Polym. J., 2022, 54, 1501-1507. DOI: https://doi.org/10.1038/s41428-022-00693-1
  9. "Methyl group configuration on acyclic threoninol nucleic acids (aTNAs) impacts supramolecular properties"
    Murayama, K.; Kashida, H.; Asanuma, H.
    Org. Biomol. Chem., 2022, 20, 4115-4122. DOI: 10.1039/d2ob00266c
    Selected as a "Front Cover"
  10. "Orthogonal amplification circuits composed of acyclic nucleic acids enable RNA detection"
    Chen, Y.; Nagao, R.; Murayama, K.; Asanuma, H.
    J. Am. Chem. Soc., 2022, 144, 5887-5892. DOI: 10.1021/jacs.1c12659
    Selected as a "Supplementary Cover"
  11. "Renewable DNA proportional-integral controller with photoresponsive molecules"
    Tamba, M.; Murayama, K.; Asanuma, H.; Nakakuki, T.
    Micromachines, 2022, 13, 193. doi.org/10.3390/mi13020193
  12. "Signal Amplification Circuit Composed of Serinol Nucleic Acid for RNA Detection"
    Chen, Y.; Murayama, K.; Asanuma, H.
    Chem. Lett., 2022, 51, 330-333. DOI:10.1246/cl.210813
  13. "Unexpected Dissociation of Photoresponsive UV-ON DNA Carrying p-tert-Butyl Azobenzene under UV Light Irradiation"
    Ishii, S.†; Murayama, K.†; Sada, K.; Asanuma, H.; Kakugo, A.
    Chem. Lett., 2022, 51, 292-295. DOI:10.1246/cl.210788
    (†; These authors contributed equally to this work.)
  14. "Pyrene modified serinol nucleic acid nanostructure converts chirality of threoninol nucleic acids into circularly polarized luminescence signals"
    Kashida, H.; Nishikawa, K.; Ito Y.; Murayama, K.; Hayashi, I.; Kakuta, T.; Ogoshi, T.; Asanuma, H.
    Chem. Eur. J., 2021, 27, 14582-14585. DOI: 10.1002/chem.202102333
    Selected as a "Cover Feature"
  15. "Light-regulated liquid-liquid phase separation for spatiotemporal protein recruitment and cell aggregation"
    Ikeuchi, N.; Komachi, T.; Murayama, K.; Asanuma, H.; Maruyama, A.; Shimada, N.
    ACS Appl. Mater. Interfaces, 2021, 13, 5652-5659. DOI: 10.1021/acsami.0c22314
  16. "Nonenzymatic polymerase-like template-directed synthesis of acyclic L-threoninol nucleic acid"
    Murayama, K.; Okita, H.; Kuriki, T.; Asanuma, H.
    Nat. Commun., 2021, 12, 804. DOI: 10.1038s41467-021-21128-0
    Selected as a featured article
  17. "Dual crosslinking photo-switches for orthogonal photo-control of hybridization between serinol nucleic acid and RNA"
    Yamano, Y.; Murayama, K.; Asanuma, H.
    Chem. Eur. J., 2021, 27, 4599-4604. doi.org/10.1002/chem.202003528
    Selected as a "Cover Feature"
  18. "Intrastrand backbone-nucleobase interactions stabilize unwound right-handed helical structures of heteroduplexes of L-aTNA/RNA and SNA/RNA"
    Kamiya, Y.; Satoh, T.; Kodama, A.; Suzuki, T.; Murayama, K.; Kashida, H.; Uchiyama, S.; Kato, K.; Asanuma, H.
    Commun. Chem., 2020, 3, 156. DOI: 10.1038/s42004-020-00400-2
  19. "Photo-regulated trajectories of gliding microtubules conjugated with DNA"
    Akter, M.; Keya, J.J.; Kabir, A.M.R.; Asanuma, H.; Murayama, K.; Sada, K.; Kakugo, A.
    Chem. Commun., 2020, 56, 7953-7956. DOI: 10.1039/d0cc03124k
  20. "A triplex-forming linear probe for sequence-specific detection of duplex DNA with high sensitivity and affinity"
    Chen, Y.; Murayama, K.; Kashida, H.; Kamiya, Y.; Asanuma, H.
    Chem. Commun., 2020, 56, 5358-5361. DOI: 10.1039/D0CC01865A
    Selected as a "Back cover"
  21. "Incorporation of Pseudo-complementary Bases 2,6-Diaminopurine and 2-Thiouracil into Serinol Nucleic Acid (SNA) to Promote SNA/RNA Hybridization"
    Kamiya, Y.; Sato, F.; Murayama, K.; Kodama, A.; Uchiyama, S.; Asanuma, H.
    Chem. Asian J., 2020, 15, 1266-1271. DOI: 10.1002/asia.201901728
    Selected as a "Cover Picture"
  22. "A Quencher‐Free Linear Probe from Serinol Nucleic Acid with a Fluorescent Uracil Analogue"
    Murayama, K.; Asanuma, H.
    ChemBioChem, 2020, 21, 120-128. DOI: 10.1002/cbic.201900498
    Selected as a "Cover Feature"
  23. "8-Pyrenylvinyl Adenine Controls Reversible Duplex Formation between Serinol Nucleic Acid and RNA by [2+2] Photocycloaddition"
    Murayama, K.; Yamano, Y.; Asanuma, H.
    J. Am. Chem. Soc., 2019, 141, 9485-9489. DOI: 10.1021/jacs.9b03267
    Selected as a "Supplementary Cover"
  24. "cis-on/trans-off of DNA hybridization with alkylthio-azobenzene on L-threoninol responding to visible light"
    Asanuma, H.; Ishikawa, T.; Yamano, Y.; Murayama, K.; Liang, X.
    ChemPhotoChem, 2019, 3, 418-424. DOI: 10.1002/cptc.201900060
    Selected as a "VIP"
    Selected as a "Cover Feature"
  25. "Orthogonally Photocontrolled Non-Autonomous DNA Walker"
    Skugor, M.; Valero, J.; Murayama, K.; Centola, M.; Asanuma, H.; Famulok, M.
    Angew. Chem. Int. Ed., 2019, 58, 6948-6951. DOI: 10.1002/anie.201901272
  26. "The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons"
    Murayama, K.; Kashida H.; Asanuma, H.
    Method in Molecular Biology, 2019, Vol. 1973, : Non-Natural Nucleic Acids.
  27. "Introduction of 2,6-diaminopurines into serinol nucleic acid (SNA) improves anti-miRNA performance"
    Kamiya, Y.; Donoshita, Y.; Kamimoto, H.; Murayama, K.; Ariyoshi, J.; Asanuma, H.
    ChemBioChem, 2017, 18, 1917-1922
  28. "Antisense oligonucleotide modified with serinol nucleic acid (SNA) induces exon skipping in mdx myotubes"
    Le, B. T.; Murayama, K.; Shabanpoor, F.; Asanuma, H.; Veedu, R. N.
    RSC Advances, 2017, 7, 34049-34052.
  29. "D-aTNA circuit orthogonal to DNA can be operated by RNA input via SNA"
    Murayama, K.; Nagao, R.; Asanuma, H.
    ChemistrySelect, 2017, 2(20), 5624-5627.
  30. "Effect of Methyl Group on Acyclic Serinol Scaffold for Tethering Dyes on DNA Duplex stability"
    Murayama, K.; Asanuma, H.
    ChemBioChem, 2017, 18, 142-149.
  31. "Strand-invading linear probe combined with unmodified PNA"
    Aasanuma, H.; Niwa, R.; Akahane, M.; Murayama, K.; Kashida, H.; Kamiya, Y.
    Bioorg. Med. Chem., 2016, 24, 4129-4137. DOI:10.1016/j.bmc.2016.06.055
  32. "Visible Light-Triggered Crosslinking of DNA Duplex by Reversible [2+2] Photocycloaddition of Styrylpyrene"
    Doi, T.; Kawai, H.; Murayama, K.; Kashida, H.; Asanuma, H.
    Chem. Eur. J., 2016, 22, 10533-10538
  33. "Azobenzene C-nucleosides for photo-controlled hybridization of DNA at room temperature"
    Goldau, T.; Murayama, K.; Brieke, C.; Asanuma, H.; Heckel, A.
    Chem. Eur. J., 2015, 21, 17870-17876, DOI : 10.1002/chem.201503303
  34. "Ultra-Sensitive Molecular Beacon Designed with Totally Serinol Nucleic Acid (SNA) for Monitoring mRNA in Cell"
    Murayama, K.; Kamiya, Y.; Kashida, H.; Asanuma, H.
    ChemBioChem, 2015, 16, 1298-1301, DOI: 10.1002/cbic.201500167
  35. "Acyclic L-Threoninol Nucleic Acid (L-aTNA) with Suitable Structural Rigidity Cross-pairs with DNA and RNA"
    Murayama, K.; Kashida, H.; Asanuma, H.
    Chem. Commun., 2015, 51, 6500-6503
    Selected as a "Back Cover"
  36. "Reversible photoswitching of RNA hybridization at room temperature with an azobenzene C-nucleoside."
    Goldau, T.†; Murayama, K.†; Brieke, C. Steinwand, S.; Mondal, P.; Biswas, M.; Burghardt, I.; Wachtveitl, J.; Asanuma, H.; Heckel, A.
    Chem. Eur. J., 2015, 21, 2845-2854. (†; These authors contributed equally to this work.)
  37. "Enhancement of stability and activity of siRNA by terminal substitution with Serinol Nucleic Acid (SNA)."
    Kamiya, Y.; Takai, J.; Ito, H.; Murayama, K.; Kashida, H.; Asanuma, H.
    ChemBioChem, 2014, 15, 2549-2555.
    Selected as a "Back Cover"
  38. "The “sugar-deficient” G-quadruplex: incorporation of aTNA in quadruplex structures."
    Zhou, J.†; Murayama, K.†; Amrane, S.; Kashida, H.; Bourdoncle, A.; Asanuma, H.; Mergny, J. L.
    Chem. Sci., 2013, 4, 3693-3698. (†; These authors contributed equally to this work.)
  39. "Highly stable duplex formation by artificial nucleic acids aTNA and SNA with acyclic scaffolds."
    Murayama, K.; Tanaka, Y.; Toda, T.; Kashida, H.; Asanuma, H.
    Chem. Eur. J., 2013, 19, 14151-14158.
  40. "Control of the Chirality and Helicity of Oligomers of Serinol Nucleic Acid (SNA) by Sequence Design"
    Kashida, H.; Murayama, K.; Toda, T.; Asanuma, H.
    Angew. Chem. Int. Ed., 2011, 50, 1285-1288.
  41. "Unexpectedly stable artificial duplex from flexible acyclic threoninol"
    Asanuma, H.; Toda, T.; Murayama, K.; Liang, X.G.; Kashida, H.
    J. Am. Chem. Soc., 2010, 132, 14702-14703.

Reviews

  1. "Highly Functional Acyclic Xeno Nucleic Acids"
    Murayama, K.; Okita, H.; Asanuma, H.
    Bull. Chem. Soc. Jpn., 2023, 96, 1179-1187. DOI: doi.org/10.1246/bcsj.20230188
  2. "Functionalization of Acyclic Xenonucleic Acid with Modified Nucleobases"
    Murayama, K.; Yamano, Y.; Asanuma, H.
    Polym. J., 2023, 55, 743-752. DOI: doi.org/10.1038/s41428-023-00776-7
  3. "Xeno nucleic acids (XNAs) having non-ribose scaffolds with unique supramolecular properties"
    Asanuma, H.; Kamiya, Y.; Kashida, H.; Murayama, K.
    Chem. Commun., 2022, 28, 3993. DOI: 10.1039/d1cc05868a
    Selected as a "Front cover"
  4. "Possibility of pre-RNA world with acyclic XNA before RNA"
    Asanuma, H.; Okita, H.; Murayama, K.
    Viva Origino, 2021, 49, 9. DOI: https://doi.org/10.50968/vivaorigino.49_9
  5. "Design and Hybridization Properties of Acyclic Xeno Nucleic Acid Oligomers"
    Murayama, K.; Asanuma, H.
    ChemBioChem, 2021, 22, 2507-2515. doi.org/10.1002/cbic.202100184
    Selected as "Cover Feature"
  6. "The DNA duplex as an aqueous one-dimensional soft crystal scaffold for photochemistry."
    Asanuma, H.; Murayama, K.; Kamiya, Y.; Kashida, H.
    Bull. Chem. Soc. Jpn., 2018, 91, 1739-1748.
  7. "Design of photo-functional oligonucleotide by "copolymerization" of natural nucleobases with base-surrogate prepared from acyclic scaffold."
    Asanuma, H.; Murayama, K.; Kamiya, Y.; Kashida, H.
    Polymer J., 2017, 49, 279-289
  8. "Acyclic artificial nucleic acids with phosphodiester bonds exhibit unique functions"
    Kashida, H., Murayama, K., Asanuma, H.
    Polymer J., 2016, 48, 781-786

Books

  1. "6.5 Orthogonality of Nucleic Acids"
    Keiji Murayama, Hiroyuki Asanuma
    "Molecular Robotics An Introduction", Satoshi Murata Ed., Springer, p.230-238. (2022)

Awards

  • Lecture Award for Encouragement of Young Researcher , 26th バイオ・高分子シンポジウム, 2016
  • Lecture Award, 13th バイオ関連化学シンポジウム 2019
  • Ohtsuka Award, (ISNAC Outstanding Oral Presentation Award for Young Scientist 2019)
  • Awards for Encouragement of Research in Polymer Science; The Society of Polymer Science, Japan 2020
  • Chemical Society of Japan Award for Young Chemists; The Chemical Society of Japan, 2022