Achievements

Achievements

Papers

Interfacial microbial engineering

S. Yoshimoto, S. Aoki, M. Ishikawa, A. Suzuki and K. Hori; Size-dependent ability of AtaA to immobilize cells in Acinetobacter sp. Tol 5, Sci. Rep. 14, 21039 (2024).
S. Yoshimoto, S. Ishii, A. Kawashiri, T. Matsushita, D. Linke, S. Göttig, VAJ. Kempf, M. Takai and K. Hori; Adhesion preference of the sticky bacterium Acinetobacter sp. Tol 5., Front. Bioeng. Biotechnol. 12, 1342418 (2024).
K. Noba, S. Yoshimoto, Y. Tanaka, T. Yokoyama, T. Matsuura, and K. Hori; Simple Method for the Creation of a Bacteria-Sized Unilamellar Liposome with Different Proteins Localized to the Respective Sides of the Membrane, ACS Synth. Biol. 12, 1437-1446 (2023).
M. Ishikawa, H. Nakatani and K. Hori; Growth phase-dependent production of the adhesive nanofiber protein AtaA in Acinetobacter sp. Tol 5, J. Biosci. Bioeng. 135, 224-231 (2023).
S. Yoshimoto, S. Aoki, Y. Ohara, M. Ishikawa, A. Suzuki, D. Linke, A. Lupas, K. Hori; Identification of the adhesive domain of AtaA from Acinetobacter sp. Tol 5 and its application in immobilizing Escherichia coli, Front. Bioeng. Biotechnol. 10, 1095057 (2023).
H. Nakatani, N. Yamada, N. Hashimoto, F. Okazaki, T. Arakawa, Y. Tamaru and K. Hori; Perturbation by Antimicrobial Bacteria of the Epidermal Bacterial Flora of Rainbow Trout in Flow-Through Aquaculture, Biology 11, 1249 (2022).
M. Ishikawa and K. Hori; Complete Genome Sequence of the Highly Adhesive Bacterium Acinetobacter sp. Strain Tol 5, Microbiol. Resour. Announc. 10, e00567-21 (2021).
S. Ishii, S. Yoshimoto and K. Hori; Single-cell adhesion force mapping of a highly sticky bacterium in liquid, J. Colloid Interface Sci. 606, 628-634 (2022).
Y. Furuichi, S. Yoshimoto, T. Inaba, N. Nomura, and K. Hori; Process Description of an Unconventional Biofilm Formation by Bacterial Cells Autoagglutinating through Sticky, Long, and Peritrichate Nanofibers, Environ. Sci. Technol. 54, 2520-2529 (2020).
K. Noba, M. Ishikawa, A. Uyeda, T. Watanabe, T. Hohsaka, S. Yoshimoto, T. Matsuura and K. Hori; Bottom-up creation of an artificial cell covered with the adhesive bacterionanofiber protein AtaA, J. Am. Chem. Soc. 141, 19058-19066 (2019).
[Cover picture]
S. Aoki, S. Yoshimoto, M. Ishikawa, D. Linke, A. Lupas and K. Hori; Native display of a huge homotrimeric protein fiber on the cell surface after precise domain deletion, J. Biosci. Bioeng. 129, 412-417 (2020).
Y. Ohara, S. Yoshimoto and K. Hori; Control of AtaA-mediated bacterial immobilization by casein hydrolysates, J. Biosci. Bioeng. 128, 544-550 (2019).
Y. Furuichi, K. Iwasaki and K. Hori; Cell behavior of the highly sticky bacterium Acinetobacter sp. Tol 5 during adhesion in laminar flows, Sci. Rep. 8, 8285 (2018).
H. Nakatani, N. Ding, Y. Ohara and K. Hori; Immobilization of Enterobacter aerogenes by a Trimeric Autotransporter Adhesin, AtaA and Its Application to Biohydrogen Production, Catalysts 8, 159 (2018).
H. Nakatani, J. Kanie and K. Hori; On‐fiber display of a functional peptide at sites distant from the cell surface using a long bacterionanofiber of a trimeric autotransporter adhesin, Biotechnol. Bioeng. 116, 239-249 (2018).
S. Yoshimoto, Y. Ohara, H. Nakatani and K. Hori; Reversible bacterial immobilization based on the salt-dependent adhesion of the bacterionanofiber protein AtaA, Microb. Cell Factories 16, 123 (2017).
J. C. Leo, P. Oberhettinger, S. Yoshimoto, D. B. R. K. G. Udatha, J. P. Morth, M. Schütz, K. Hori and D. Linke; Secretion of the Intimin Passenger Domain Is Driven by Protein Folding, J. Biol. Chem. 291, 20096-20112 (2016).
S. Yoshimoto, H. Nakatani, K. Iwasaki and K. Hori; An Acinetobacter trimeric autotranspoter adhesin reaped from cells exhibits its nonspecific stickiness via a highly stable 3D strucuture, Sci. Rep. 6, 28020 (2016).
M. Ishikawa, S. Yoshimoto, A. Hayashi, J. Kanie and K.Hori; Discovery of a novel periplasmic protein that forms acomplex with a trimeric autotransporter adhesin and Peptidoglycan, Mol. Microbiol. 101, 394-410 (2016).
K. Koiwai, M. D. Hartmann, D. Linke, A. N. Lupas and K. Hori; Structural basis for toughness and flexibility in the C-terminal Passenger domain of an Acinetobacter trimeric autotranspoter Adhesin, J. Biol. Chem. 291, 3705-3724 (2016).
K. Hori, Y. Ohara, M. Ishikawa and H. Nakatani; Effectiveness of direct immobilization of bacterial cells onto material surfaces using the bacterionanofiber protein AtaA, Appl. Microbiol. Biotechnol. 99, 5025-5032 (2015).
[Spotlighted Article]
M. Ishikawa, K. Shigemori and K. Hori; Application of the adhesive bacterionanofiber AtaA to a novel microbial immobilization method for the production of indigo as a model chemical, Biotechnol. Bioeng. 111, 16-24 (2014).
H. Liu, M. Ishikawa, S. Matsuda, Y. Kimoto, K. Hori, K. Hashimoto and S. Nakanishi; Extracellular electron transfer of a highly adhesive and metabolically versatile bacterium, ChemPhysChem 14, 2407-12 (2013).
M. Ishikawa and K. Hori; A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination, BMC Microbiol. 13, 86 (2013).
M. Ishikawa, H. Nakatani, K. Hori; AtaA, a New Member of the Trimeric Autotransporter Adhesins from Acinetobacter sp. Tol 5 Mediating High Adhesiveness to Various Abiotic Surfaces, PLoS One 7, e48830 (2012).
S. Matsumoto, A. Ohtaki and K. Hori; Carbon Fiber as an Excellent Support Material for Wastewater Treatment Biofilms, Environ. Sci. Technol. 46, 10175-181 (2012).
M. Ishikawa, K. Shigemori, A. Suzuki and K. Hori; Evaluation of adhesiveness of Acinetobacter sp. Tol 5 to abiotic surfaces, J. Biosci. Bioeng. 113, 719-725 (2012).
K. Hori, M. Ishikawa, M. Yamada, A. Higuchi, Y. Ishikawa, and H. Ebi; Production of peritrichate bacterionanofibers and their proteinaceous components by Acinetobacter sp. Tol 5 cell affected by growth substrates, J. Biosci. Bioeng. 111, 31-36 (2010).
K. Hori, N. Hiramatsu, M. Nannbu, K. Kanie, M. Okochi, H. Honda, and H. Watanabe; Drastic change in cell surface hydrophobicity of a new bacterial strain, Pseudomonas sp. TIS1-127, induced by growth temperature and its effects on the toluene-conversion rate, J.Biosci. Bioeng. 107, 250-255 (2009).
H. Watanabe, Y. Tanji, H. Unno, K. Hori; Rapid conversion of toluene by an Acinetobacter sp. Tol 5 mutant showing monolayer adsorption to oil-water interface, J. Biosci. Bioeng. 106, 226-230 (2008).
K. Hori, H. Watanabe, S. Ishii, Y. Tanji, and H. Unno; Monolayer Adsorption of a “Bald” Mutant of the Highly Adhesive and Hydrophobic Bacterium Acinetobacter sp. Strain Tol 5 to a Hydrocarbon Surface, Appl. Environ. Microbiol. 74, 2511-2517 (2008).
S. Ishii, S. Miyata, Y. Hotta, K. Yamamoto, H. Unno, and K. Hori; Formation of filamentous appendages by Acinetobacter sp. Tol 5 to adhere to solid surfaces, J. Biosci. Bioeng. 105, 20-25 (2008).
S. Ishii, H. Unno, S. Miyata, K. Hori; Effect of cell appendages on the adhesion property of a highly adhesive bacterium, Acinetobacter sp. Tol 5, Biosci. Biotech. Biochem. 70, 2635-2640 (2006).
S. Ishii, T. Kosaka, K. Hori, Y. Hotta, and K. Watanabe; Coaggregation Facilitates Interspecies Hydrogen Transfer between Pelotomaculum thermopropionicum and Methanothermobacter thermautotrophicus, Appl. Environ. Microbiol. 71, 7838-7845 (2005).

Wastewater treatment and bioremediation

S. Takahashi and K. Hori; Long-term continuous degradation of carbon nanotubes by a bacteria-driven Fenton reaction, Front. Microbiol. 14, 1298323 (2023)
S. Takahashi, F. Taguchi, and K. Hori; Contribution of the Fenton reaction to the degradation of carbon nanotubes by enzymes, Front. Environ. Sci. 11, 1184257 (2023)
Y.-Y. Chen, M. Ishikawa, and K. Hori; A novel inverse membrane bioreactor for efficient bioconversion from methane gas to liquid methanol using a microbial gas-phase reaction, Biotechnol. Biofuels 16, 16 (2023).
M. Ishikawa, T. Kojima, and K. Hori; Development of a Biocontained Toluene-Degrading Bacterium for Environmental Protection, Microbiol. Spectr. 9, e00259-21 (2021).
Y.-Y. Chen, Y. Soma, M. Ishikawa, M. Takahashi, Y. Izumi, T. Bamba and K. Hori; Metabolic alteration of Methylococcus capsulatus str. Bath during a microbial gas-phase reaction, Biores. Technol. 330, 125002 (2021).
H. Nakatani and K. Hori; Establishing a Percutaneous Infection Model Using Zebrafish and a Salmon Pathogen, Biology 10, 166 (2021).
Y.-Y. Chen, M. Ishikawa, R, Suzuki, H. Ito, T. Kamachi and K. Hori; Evaluation of methane degradation performance in microbial gas-phase reactions using effectively immobilized methanotrophs, Biochem. Eng. J. 154, 107441 (2020).
A. Usami, M. Ishikawa and K. Hori; Gas-phase bioproduction of a high-value-added monoterpenoid (E)-geranic acid by metabolically engineered Acinetobacter sp. Tol 5, Green Chem. 22, 1258-1268 (2020).
M. Ishikawa, S. Yokoe, S. Kato, and K. Hori; Efficient Counterselection for Methylococcus capsulatus (Bath) by Using a Mutated pheS Gene, Appl. Environ. Microbiol. 84, e01875-18 (2018).
K. Tanaka, S. Yokoe, K. Igarashi, M. Takashino, M. Ishikawa, K. Hori, S. Nakanishi, and S. Kato; Extracellular Electron Transfer via Outer Membrane Cytochromes in a Methanotrophic Bacterium Methylococcus capsulatus (Bath), Front. Microbiol. 9, 2905 (2018).
M. Ishikawa, Y. Tanaka, R. Suzuki, K. Kimura, K. Tanaka, K. Kamiya, H. Ito, S. Kato, T. Kamachi, K. Hori, and S. Nakanishi; Real-time monitoring of intracellular redox changes in Methylococcus capsulatus (Bath) for efficient bioconversion of methane to methanol, Biores. Technol. 241, 1157-1161. (2017)
D. Okamura, Y. Mori, T. Hashimoto, and K. Hori; Effects of microbial degradation of biofoulants on microfiltration membrane performance in a membrane bioreactor, Environ. Sci. Technol. 44, 8644-8648 (2010).
D. Okamura, Y. Mori, T. Hashimoto, and K. Hori; Identification of biofoulant of membrane bioreactors insoluble microbial products, Water Res. 43, 4356-4362 (2009).
H. Matsuoka, A. Miura, and K. Hori; Symbiotic effects of a lipase-secreting bacterium, Burkholderia arboris SL1B1, and a glycerol-assimilating yeast, Candida cylindracea SL1B2, on triacylglycerol degradation, J. Biosci. Bioeng. 107, 401-408 (2009).
K. Miyanaga, S. Takano, Y. Morono, K. Hori, H. Unno and Y. Tanji; Optimization of distinction between viable and dead cells by fluorescent staining method and its application to bacterial consortia, Biochem. Eng. J. 37, 56-61 (2007).
Y. Morono, H. Unno, K. Hori; Correlation of TCE cometabolism with growth characteristics on aromatic substrates in toluene-degrading bacteria, Biochem. Eng. J. 31, 173-179 (2006).
K. Hori, J. Mii, Y. Morono, Y. Tanji, and H. Unno; Kinetic analyses of trichloroethylene cometabolism by toluene-degrading bacteria harboring a tod homologous gene, Biochem. Eng. J. 26, 59-64.5 (2005).

Environmental biotechnology

K. Sugimoto, K. Hori, M. Ishikawa, H. Ito, T. Kamachi, K. Tanaka, Y.-Y. Chen, S. Nakanishi; Electrochemical Monitoring of Metabolic Activity of Methane/Methanol Conversing Methylococcus Capsulatus (Bath) Cells Based on Extracellular Electron Transfer, Electrochemistry 92. 022007 (2024).
H. Ito, K. Yoshimori, M. Ishikawa, K. Hori and T. Kamachi; Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant, Front. Microbiol. 12. 639266 (2021).
K. Hori, R. Ichinohe, H. Unno, and S. Marsudi; Simultaneous syntheses of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa IFO3924 at various temperatures and from various fatty acids, Biochem. Eng. J. 53, 196-202 (2011).
K. Hori, M. Abe, and H. Unno; Production of triacylglycerol and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by the toluene-degrading bacterium Rhodococcus aetherivorans IAR1, J. Biosci. Bioeng. 108, 319-324 (2009).
K. Hori, A. Kobayashi, H. Ikeda, H. Unno; Rhodococcus aetherivorans IAR1, a new bacterial strain synthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from toluene, J. Biosci. Bioeng. 107, 145-150 (2009).
S. Marsudi, H. Unno, K. Hori; Palm oil utilization for simultaneous production of polyhydroxyalkanaotes and rhamnolipids by Pseudomonas aeruginosa, Appl. Microbiol. Biotechnol. 78, 955-961 (2008).
B-I. Sang, W-K. Lee, K. Hori, and H. Unno; Purification and Characterization of Fungal Poly(3-hydroxybutyrate) Depolymerase from Paecilomyces lilacinus F4-5 and Enzymatic Degradation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Film, World J. Microbiol. Biotechnol. 22, 51-57 (2006).

Other Papers

Y. Ohara, J. Kanie, and K. Hori; Fabrication of a highly protective 3D-printed mask and evaluation of its viral filtration efficiency using a human head mannequin, HardwareX 11, e00314 (2022).
[Cover picture]
A. Usami, M. Ishikawa, and K. Hori; Heterologous expression of geraniol dehydrogenase for identifying the metabolic pathways involved in the biotransformation of citral by Acinetobacter sp. Tol 5, Biosci. Biotechnol. Biochem. 82, 2012-2020 (2018).
K. Koiwai,T. Kubota, N. Watanabe, K. Hori, O. Koiwai and H. Masai; Definition of the transcription factor TdIF1 consensus-binding sequence through genomewide mapping of its binding sites, Genes Cells 20, 242-254 (2015).
T. Kubota, O. Koiwai, K. Hori, N. Watanabe, K. Koiwai; TdiF1 recognizes a specific DNA sequence through its helix-turn-helix and AT-hook motifs to regulate gene transcription, PLoS One 8, e66710 (2013).

Reviews

K. Hori, S. Yoshimoto, T. Yoshino, T. Zako, G. Hirao, S. Fujita, C. Nakamura, A. Yamagishi, N. Kamiya; Recent advances in research on biointerfaces: From cell surfaces to artificial interfaces, J. Biosci. Bioeng. 133, 195-207 (2022).
[Invited Review Paper]
H. Nakatani and K. Hori; Cell surface protein engineering for high-performance whole-cell catalysts, Front. Chem. Sci. Eng. 11, 46-57 (2017).
[Invited Review Paper]
K. Hori, and S. Matsumoto; Bacterial adhesion: From mechanism to control, Biochem. Eng. J. 48, 424-434 (2010).

Books

K. Hori, H. Unno; Integrated Production and Separation, Comprehensive Biotechnology(Third Edition), p651-662 (2019).
K. Hori and H. Unno; Integrated production and separation, In Reference Module in Life Sciences, Elsevier, (2017)
K. Hori; Biofilm and Materials Science: Adhesion of Bacteria; Springer, p23-34 (2015).
K. Hori and H. Unno; Bioreactions and Bioreactor Operation | Integrated production and separation, in Murray Moo-Young (ed.), “Comprehensive Biotechnology”, Second Edition, volume 2, Elsevier, p579-590 (2011).